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KMID : 1059520190630060420
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Journal of the Korean Chemical Society
2019 Volume.63 No. 6 p.420 ~ p.426
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A Study for the Relation Between Selenoproteins and Korean Rectal Cancer Using Deuterium Collision Gas HPLC-ICP/MS
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Lee Seo-Young
Pak Yong-Nam
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Abstract
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Selenoproteins,in Korean blood serum, GPx, SelP, and SeAlb were separated and determined with the use of HPLC-ICP/MS. Deuterium was used as a collision gas and affinity column with ammonium formate was used as an eluting solvent for the accurate quantitation of selenoproteins in human blood serum. Certified reference material BCR 639 (133±12 ng g-1) was tested for the accuracy and the result was satisfactory 130±6 ng g-1. Blood serum for the rectal cancer and controlled groups were collected and analyzed to give 84±27 ng g-1, and 119±28 ng g-1, respectively. The difference was statistically obvious when t-test was performed (tcal 4.93 > t95% 2.04). The decrease for cancer group was more obvious for female and aged group. The distributions of three selenoproteins were similar with each other, which means rectal cancer group did not show any specificity for any selenoproteins. As cancer developed, GPx showed a slight decrease but not obvious while the total concentration was increasing particularly at the second stage of cancer.
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KEYWORD
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Selenium, Selenoprotein, Rectal cancer, Blood serum, HPLC-ICP/MS
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